FT3 Test ( 1 )

Thyroid gland synthesizes and secretes Triiodothyronine.T3 is formed by peripheral deiodination of thyroxine(T4).Thyroid stimulating hormone (TSH) leads to secretion of T3 and T4 into circulation and plays an important role in regulating metabolism. Secretion of T3 and T4 is regulated by a negative feedback mechanism involving the thyroid gland, and hypothalamus. There is reversible binding of 99.95% of T3 to transport proteins, primarily thyroxine-binding globulin (TBG and to some extent with albumin and pre-albumin. Rest of the T3 is free in the circulation and does not attach to transport proteins.This unattached fraction of the total T3 concentration is free triiodothyronine (free T3). There is a direct correlation of T3 levels with T3 secretion and metabolism.We have provide ft3 test cost at a discounted price.

Test Principle 

This test is based on the principle of chemiluminescence immunoassay (CLIA). Test consists of two steps, first step involves combining assay diluent and anti-FT3 coated paramagnetic microparticles with the test sample. Binding takes place between anti- FT3 coated microparticles and FT3 present in the sample. This is followed by washing step and addition of acridinium-labeled anti-FT3 conjugate to the combined mixture. Second washing is preformed, followed by addition of pre-trigger and trigger solutions are added to the reaction mixture.

The resulting chemiluminescent reaction is measured in relative light units {RLUs). There exists a direct relationship between the amount of FT3 in the sample and the RLUs detected. Tire presence or absence of FT3 in the sample is determined by- comparing the chemiluminescent signal in the reaction to the cutoff signal determined.

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